Cucumis sativus (Cucumber) is a widely cultivated plant (Family: Cucurbitaceae). It is a creeping vine that bears cucumiform, fruits that are used as vegetables. Traditionally, Cucumis sativus possesses anti-diarrhoeal, anti-urolithiatic, anti-inflammatory, anti-hypertensive, proteolytic, anti-fungal, antioxidant and anti panic activities. The leaves of this plant is used for headache, seeds as diuretic, the fruit juice of this plant is used as nutritive and as a demulcent in anti-acne lotions. In the present study the ethanolic leaf extract of Cucumis sativus is screened for its phytochemical and biological activity (anti-panic activity) as per the traditional value by scientific manner. The results concluded that the ethanolic leaf extract of Cucumis sativus possessed significant anti-panicactivity when compared with the standard diazepam.