Cytotoxic potential of the phytoconstituents extracted from leaves of Uvaria narum (Dunal) Wall. ex Hook.f. and Thoms was evaluated.Biochemical and thin layer chromatography (TLC) analysis revealed phytosterols, terpenoids, flavonoids, phenols and carbohydrates in various solvent extracts. Phytosterols and terpenoids were the major constituents in petroleum ether (PE) and chloroform (CHL) extracts. Cytotoxic effect of individual extractonDalton's Lymphoma Ascites (DLA), Ehrlich's Ascites Carcinoma (EAC) and normal rat spleen cells were determined by trypan blue exclusion method. Towards these cells, PE, CHL, Acetone (ACT) and Methanol (MeOH) extracts showed significant toxic effect. However the aqueous extract was non toxic. The cytotoxicity displayed by PE extract was selective being toxic to cancer cells (IC50 for DLA was 19 ± 0.57 and that of EAC cells was 38 ± 0.74 µg/mL) while less toxic to normal spleen cells (Above 100 µg/mL).The study indicates the presence of cytotoxic phytochemicals in U.narum leaf with remarkable potential in the chemotherapeutic drug development.
Indigofera Suffruticosais an Indian herb used for various ailments by traditional healers and it is a small tree plant in the family Fabaceae, traditionally used in treatment of several diseases (fever, headaches, hemorrhages, convulsions, acute cough and skin parasites).The present study was carried out to investigate the phytochemical profile of leaves of Indigofera Suffruticosa. The powder of the leaves of Indigofera Suffruticosawas successively extracted with acetone, benzene, chloroform, cyclohexane, ethylacetate and methanol. Phytochemical analysis shows the presence of carbohydrates, glycosides, proteins, amino acids, phenolic compounds, tannins, steroids, anthraquinone, anthocyanin, flavonoid, and alkaloid. The result of the study could be useful to description and foundation of monograph of the plant.
The food borne diseases is rapidly changing their epidemiology. Recently described pathogens, such as Escherichia coli and the epidemic strain of Salmonella serotype Typhimurium Definitive Type 104 have become important public health problems. Well- recognized pathogens, such as Salmonella serotype Enteritidis, have increased in prevalence or become associated with new vehicles. Emergence in food borne diseases is driven by the same forces as emergence in other infectious diseases: changes in demographic characteristics, human behavior, industry, and technology; the shift toward a global economy; microbial adaptation; and the breakdown in the public health infrastructure. Addressing emerging food borne diseases will require more sensitive and enhanced methods of laboratory identification and sub typing, and effective prevention and control. The epidemiology of food borne disease is changing. New pathogens have the contamination of human food with sewage or animal manure.
The present study was carried out to investigate the influence of Seaweed Liquid Fertilizer (SLF) of the brown seaweed Colpomenia sinuosa(Mert. ex Roth) Derbes & Solieron the seed germination, shoot length, root length, biochemical contents and pigment characteristics of Pennisetum glaucum (L.) R.Br.Experiments were conducted on Pennisetum glaucum (L.) R.Br.to study the potential brown seaweed Colpomenia sinuosa(Mert. ex Roth) Derbes & Solieras a biofertilizer. The Seaweed Liquid Fertilizer was prepared in four different concentrations separately (2.5%, 5.0%, 7.5% and 10%). The 10% concentration of SLF showed the best positive results on seed germination, shoot length, root length, biochemicals such as total carbohydrates, total proteins, total lipids, total phenols and pigments namely total chlorophylls, total caraotenoids. The data generated from the present study reveal that the Seaweed Liquid Fertilizer of Colpomenia sinuosa(Mert. ex Roth) Derbes & Solier can be used as biofertilizer to increase the product ofPennisetum glaucum (L.) R.Br.
Various traditional systems of medicine enlightened the importance of Indian plants to have a great medicinal value. The present study was aimed to evaluate the antimicrobial activity of Vetiveria lawsonii(Family-Poaceae). The benzene extract was prepared by Soxhlet extraction. The preliminary phytochemical investigation revealed the presence of Alkaloids, Terpenoids, Steroids, and Saponins. The benzene extract of Vetiveria lawsonii showing high antimicrobial activity against the microbes revealed by Agar well diffusion method. Hence, we can conclude that the benzene extracts of Vetiveria lawsonii was possess antimicrobial activity.
Cellulite is an aesthetic problem which occurs in 85% of Asian women over the age of 20 years and most often found in the area of the thigh, buttock and stomach, which its treatment is expensive and can cause many side effects. The objective of this experiment was to determine the effect of the mixture of essential oil of patchouli (Pogostemoncablin), jasmine (Jasminumsambac) and ginger (Zingiberofficinale) in reducing the sign of cellulite. The efficacy test was conducted using open label comparative clinical trial with inter-subject design, single blind on 21 women subjects with cellulite of1-3 degrees. The mixture containing essential oils with a concentration of 1% each was applied to subject’s skin for 28 days and observed for the irritation symptoms and effects on cellulite by circumference measurements, skin roughness (depth of wrinkles) and photography. The results showed that the mixture did not irritate the skin and significantly reduce the thigh circumference measurements and skin roughness (p<0.05).The photographic data showed that there was an improvement in skin surface appearance, although the degree of cellulite has not been changed.
Luh Mas Sukeningsih*, Anna S. Ranti, Endang Hanani.
The advent of highly active antiretroviral therapy (HAART) has decreased mortality and improved the quality of life for HIV positive people, but treatment of HIV and its associated conditions remains highly complex, With some antiretroviral agents, dozens of drugs for opportunistic illnesses (OIs), and additional therapies to manage associated conditions such as elevated blood fats, the potential for drug interactions is a pressing concern. The increasing complexity of medicine in developed societies today also carries an increased risk of interactions leading to reduced efficacy or to toxic reactions. Which anti -retrovirals should not be used with other drugs. HIV positive people need not despair if they must take a medication implicated in many interactions. Often, drug interactions can be overcome simply by raising or lowering doses however, this should never be done without the guidance of a knowledgeable practitioner. In other cases, it may be possible to replace an interacting drug with a no interacting agent that works comparably well. The problem with such lists, especially in a rapidly developing area, is that they can date quickly and should always be used with care.
K. Ragavan*, T. Haritha, G. Vanitha, P. Himabindu, M. Tejaswini, M. Niranjan Babu.
Plant of Phyllanthus niruri, an indigenous plant of India, had been the plant of study for the current research work. Aqueous as well as ethanolic extracts of Phyllanthus niruri were evaluated for their analgesic as well as anti-pyretic effect using Tail Immersion Model and Brewer’s Yeast Induced Pyrexia Model respectively. Aqueous as well as Ethanolic plant extracts induced better analgesia and have anti-pyretic potential when compared to standard drugs. Combination of analgesia as well as anti-pyretic effect will ascertain its significant role in infection induced fever.
Akila Elias*, S. Ravichandran, S. L. Neethu, S. Sumithra, S. Safeera, M. Venkadesh.
The aim of this study was to assess the effect of Tragia plukenetii R. Smith leaf extracts on the electrical threshold and its influence on the acetic acid induced writhing in mice. The preclinical evaluation of standardized benzene, chloroform, and methanolic extracts of the leaves of Tragia plukenetii R. smith was carried outfor analgesic activity using acetic acid induced writhing method in swiss albino mice. The methanolic leaf extract of Tragia plukenetii R. Smith has shown significant analgesic activity when compared with all the other groups using acetic acid induced writhing method. The results conclusively demonstrate the efficacy of Tragia plukenetii R. Smith methanolic leaf extracts for peripheral analgesic activity.
Y. Sarath Kumar*, P. Chinna, K. Ashok Kumar, M. Sathish Kumar.
Haravey reported the Aegle marmelos leaves to posses cardiotonic property, butnot much is known about the chemical constituent responsible nor the mechanism involved; in this regard the present study is a preliminary attempt to identify some of the cationic salt constituents (sodium, potassium, calcium and magnesium) of the Aqueous and Alcoholic extracts involved in the regulation of cardiotonic activity using Atomic Absorption Spectrophotometer, determination of phytochemicals and the extracts chemical analysis using HPTLC. Our study results showed variation in cationic salts composition in both the extracts but in the same order Ca>Mg>K>Na. The alcohol extract was positive for flavanoids, glycosides, tannins and saponins; while the aqueous extract was negative for flavanoids but positive for glycosides, alkaloids, tannins and saponins.