Phyto-medicines have been used traditionally by herbalists and indigenous healers worldwide for the prevention and treatment of liver diseases specially jaundice. More than sixty percent of jaundice patients of our country have the 1^st choice to visit to traditional healers for their treatment. Classical text of Ayurveda vividly narrated about Kamala (Jaundice) and many classical formulations are in active practice along with anupana (adjuvents)). Hepatitis is one of the causes for Kamala (Jaundice). None of the classical Ayurveda formulation’s therapeutic outcome is studied meticulously in hepatitis infections. Therefore we plan to observe the outcome of hepatitis cases treated with conventional Ayurveda treatment. Here we presented one hepatitis B infection treated with two classical Ayurvedic formulations, Argyavardhinivati along with leaf juice of Bhumyamlaki (Phyllanthusfraternus G.B.Wbster.) and triphlachurna. The loss of HBsAg has taken place in 45days. SGOT and SGPT were normalised after 45days of treatment. It is very clear from this observational study that Argyavardhinivati along with leaf juice of Bhumyamlaki (Phyllanthusfratarnus L.) and Triphlachurna have significant role to clearance of HBs Ag rapidly and normalise Liver Transminase in Hepatitis B infection within 45days. RCT on large sample is recommended.

The soil samples were collected from the polythene and plastic waste dumped site. The collected samples were subjected to serial dilution and plating technique. The isolated microbial strains were identified based on their cultural morphological and biochemical study. It was concluded that B.subtilis(I), B.subtilis(II), and B.megaterium degrade the polythene and plastic materials effectively. The infected leaves of Abelmoshus esculentus, Solanum melongena and Solanum lycopersicum were collected and the bacterial phytopathogens Pseudomonas aeruginosa, Pseudomonas solanacearum and Xanthomonus vesicatoria were isolated and identified through biochemical tests. The antagonistic effect of isolated plastic degrading microbes against bacterial phytopathogens was analyzed by zone of inhibition method. The functional groups were identified through IR. From this present study we concluded that the plastic degrading microbes can control the bacterial phytopathogens without any effects in plant.

The Aegle marmelos plant belonging to the family Rutaceae occupies a distinct position in Indian traditional medicine system for its medicinal properties. The chronic oral treatment study of whole leaf powder (2000mg), aqueous and alcohol extract (400mg) for 90 days in male Wistar rats showed absence of any alterations in tissue lipid peroxidation indicating the absence of any pro-oxidant property. Catalase in the liver and lung was found to be elevated during treatment with Aegle marmelos. Tissue glutathione peroxidase is found to be comparatively more in alcohol extract treated group in all the tissues analyzed. The non-enzymic antioxidants GSH and vitamin C in tissues were more after treatment with alcohol extract. Thus a desirable effect on both enzymic and non-enzymic antioxidants by leaf powder extracts of Aegle marmelos was seen in most of the tissues studied. Tissue ATPases analyzed in the present study was found to be altered during treatment with the alcohol extract. The aqueous extract and leaf powder have shown the effect only to a little extent in certain tissues. All the ATPases analyzed in brain, liver and lung increased during treatment with alcohol extract. While the alcohol extract increased the Na⁺-K⁺-ATPase alone, the aqueous extract increased all the ATPases in heart. When the relative percent increase of the enzyme activity is considered, the aqueous extract is found to enhance the activity of Ca₂⁺-ATPases more than that of Na⁺-K⁺-ATPases. In renal tissue, an increase in Ca₂⁺-ATPase was seen after aqueous extract treatment. The relevance of the possible enhanced reabsorption of calcium needs to be investigated and from the pharmacological effects identified this study needs further evaluation for their clinical significance as the leaf of Aegle marmelos is found to have a multitude of pharmacological effects without any toxic impact during long-term administration.

A simple, rapid, selective and quantitative HPTLC method has been developed for determination of Plumbagin in Ayurvedic formulations of Chitrak haritaki of different manufactures. The alcoholic extract of Chitrak Haritaki and Chitrak Root samples were applied on TLC Aluminum plate pre coated with Silicagel60 GF254 and developed using Toluene: Ethyl acetate (3:1) V/Vas a mobile phase. The plate was sprayed (derivatized) with Anisaldehyde‐Sulphuric Acid reagent followed by heating at 110⁰C for 10 minutes and detection and quantification were carried out densitometrically using an UV detector at wavelength of 254 nm. Content of marker compound in the samples were found similar.

Chemical and physical investigation of methanolic extract of roots of Solanum xanthocarpum was carried out. By phytochemical screening the presence of alkaloid, triterpenoid, phenols, tannins, flavanoids, carbohydrates, phytosterols, fats and fixed oil were confirmed. Column chromatography was performed and a compound was isolated.  The extract was estimated for their total phenolic content. Total phenolic content was found to be 76μg. Total flavonoid content was found to be 73μg. Antioxidant study was carried out on methanolic extract. It showed good % DPPH radical scavenging activity. IC50 value of the compound was found to be 52μg/ml.